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Marburg GmbH
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Korean Cell Line Bank
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Image Search Results
Journal: Cancer Research Communications
Article Title: Epigenetic Priming by Hypomethylation Enhances the Immunogenic Potential of Tolinapant in T-cell Lymphoma
doi: 10.1158/2767-9764.CRC-23-0415
Figure Lengend Snippet: Effect of necrosome protein expression in TCL cell lines on tolinapant-induced cell death. A, Western blots of parental BW5147, RIPK3 − / − BW5147 and MLKL − / − BW5147 cell lysates (untreated). B, Cytotox-NIR signal captured by real-time microscopy (IncuCyte), detecting membrane permeabilization during lytic cell death after treatment of parental BW5147, RIPK3 − / − BW5147 and MLKL − / − BW5147 cells with tolinapant in the absence or presence of zVAD. C, Western blots of Karpas-299 cells transduced with control gRNA (KARPAS-299-CTR gRNA) or with RIPK3 gRNA (KARPAS-299-RIPK3 gRNA) constructs treated with tolinapant ± zVAD for 0, 2, or 6 hours. D, Real-time microscopy (IncuCyte) measurement of membrane permeabilization by measuring Cytotox-NIR signal after treatment of KARPAS-299-CTR gRNA or KARPAS-299-RIPK3 gRNA cells with tolinapant. E, Western blots of EL4-PAR and CASP8 KO EL4 (EL4-C8KO) cell lysates after 24-hour treatment with tolinapant. F, Real-time microscopy (IncuCyte) measurement of membrane permeabilization by measuring Cytotox-NIR signal after treatment of EL4-PAR or EL4-C8KO cells with tolinapant.
Article Snippet:
Techniques: Expressing, Western Blot, Microscopy, Membrane, Transduction, Control, Construct
Journal: Cancer Research Communications
Article Title: Epigenetic Priming by Hypomethylation Enhances the Immunogenic Potential of Tolinapant in T-cell Lymphoma
doi: 10.1158/2767-9764.CRC-23-0415
Figure Lengend Snippet: Dosing with a combination of tolinapant plus decitabine drives increased efficacy in necroptosis-model of TCL (EL4-C8KO model). Volumes of EL4-PAR ( A ) and C8KO ( B ) EL4 tumors in wild-type mice treated with decitabine (0.3 mg/kg i.p., every day × 3 doses) and tolinapant dosing (25 mg/kg orally, every day × up to 46 doses) as single agents or in combination. C, Kaplan–Meier plot showing survival (tumors reaching 1,000 mm 3 as endpoint) of mice bearing EL4-C8KO (C8KO) tumors.
Article Snippet:
Techniques: